1：For Heparin applications, Sepax offer solutions for SECseparations (under USP and EP conditions) as well asimpurity analyses by IEX using Sepax’s Glycomix SAX(also under USP conditions).
2： Sepax’s SEC columns come in a range of pore sizes togive the bestseparation of the MW range for thesetypes of samples.
3：We have shown that running two Zenix SEC-100 7.8x300mm columns can further increase the separationbetween MW species since resolution is correlated tocolumn length.
4：Sepax’s Glycomix SAX was specifically developed toidentify any impurities in Heparin Samples, as shown byour separation between Dermatan Sulfate, an unknownimpurity, Oversulfated Chondroiton Sulfate and Heparin.
Heparin Analysis on SEC and SAX
LMW Heparin-Dalteparin and Enoxaparin Analysis onZenix ™ -100 (7830)
Columns: Zenix™-100 (3 ?m, 100 ?, 7.8x300 mm)
Flow Rate:
phase:
0.5 mL/min
2.84% Na 2 SO 4 , pH 5.0
Temperature: 35 o C
Detection: UV 234 nm and RI
Injection volume: 25 µL
Samples: 10 mg/mL Dalteparin in water, pH 6.0, MW 3,000 – 8,000 D
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