Anti-EMR1/FITC抗體使用說明書

Catalog Number : BYK-7058R-FITC      
Quantity size :   100ug (2mg/1ml, Lyophilized or Liquid. Buffer = 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide. Reconstitute with 50ul sterile distilled water.)

Background:  The epidermal growth factor (EGF)-TM7 family constitutes a group of class B G-protein coupled receptors, which includes CD97, EMR1 (EGF-like molecule containing mucin-like hormone receptor 1, designated F4/80 in mouse), EMR2, EMR3, FIRE, and ETL (1–3). These family members are characterized by an extended extracellular region with several N-terminal EGF domains, and are predominantly expressed on cells of the immune system (1–3). The EGF-TM7 protein family are encoded by a gene cluster on human chromosome 19p13 (1,3,4). The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria, and Langerhans cells in the skin (1). F4/80/EMR1 undergoes extensive N-linked glycosylation as well as some O-linked glycosylation (5,6). The function of F4/80/EMR1 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration, or as a G-protein coupled signaling component of macrophages.

Also known as:   F4/80; Cell surface glycoprotein EMR1; Cell surface glycoprotein F4/80; DD7A5 7; Egf like module containing mucin like hormone receptor like 1; Egf like module containing mucin like hormone receptor like sequence 1; EGF like module receptor 1; EGF TM7; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGFTM7; EMR 1; EMR1; EMR-1; EMR1 hormone receptor; EMR1_HUMAN; Gpf480; Ly71; Lymphocyte antigen 71; TM7LN3.

Specificity:   -Rabbit Polyclonal IgG, affinity purified by Protein A.

-FITC conjugated

-Reacts with: Human, Mouse, Rat, Pig, Guinea Pig

-Immunogen: KLH conjugated synthetic peptide derived from human EMR1/Gpf480.

-Predicted molecular weight: 95kDa

Application:    Flow-Cyt=1:50-200 IF=1:50-200

               ICC=1:50-200

              Excitation spectrum: 490-495nm

              Emission spectrum: 520-530nm

              Not yet tested in other applications.

              Optimal working dilutions must be determined by the end user.

Storage:      Store at -20 °C for one year. Avoid repeated freeze/thaw

             cycles. The Lyophilized or Liquid antibody is stable at room

             temperature for at least one month and for greater than a year when

             kept at -20 °C. When reconstituted in sterile pH 7.4 0.01M PBS or

             diluent of antibody the antibody is stable for at least two weeks at

             2-4 °C.

Important Note: This product as supplied is intended for research

              use only, not for use in human, therapeutic or diagnostic

              applications..