甲基化CpG結(jié)合蛋白抗體,Anti-MBD1/PCM1抗體Background: DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain （MBD）. The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 （PCM1） are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 （also known as MED1） were later found to colocalize with foci of heavily methylated salite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsalite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.

甲基化CpG結(jié)合蛋白抗體,Anti-MBD1/PCM1抗體Specificity :
Anti- MBD1/PCM1 is a rabbit polyclonal antibody unconjugated
use for western blotting,elisa,immunoprecipitation and immunohistochemistry
Protein G affinity chromatography purification, purity :>95%
Isotype: IgG
mol wt: 60kDa

甲基化CpG結(jié)合蛋白抗體,Anti-MBD1/PCM1抗體Application :
Western blotting 1:100-500
Immunohistochemistry 1:100-500
ELISA 1:500-1000
Optimal working dilutions must be determined by the end user.

Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.