Anti-Intersectin 1抗體；交叉蛋白1抗體簡單介紹：

產(chǎn)品編號：BYK-18171R

英文名稱：Anti-Intersectin 1
中文名稱：交叉蛋白1抗體

別名：Human intersectin SH3 domain containing protein SH3P17; Intersectin 1 SH3 domain protein; intersectin 1 short form variant 3; intersectin 1 short form variant, 11; Intersectin short form; intersectin short variant 12; Intersectin-1; Intersectin1; ITSN 1; ITSN; itsn1; ITSN1_HUMAN; MGC134948; MGC134949; OTTHUMP00000067855; OTTHUMP00000067856; OTTHUMP00000067857; OTTHUMP00000194810; OTTHUMP00000194811; OTTHUMP00000194812; OTTHUMP00000206688; SH3 domain containing protein 1A; SH3 domain protein 1A; SH3 domain-containing protein 1A; SH3D1A; SH3P17; Src homology 3 domain containing protein.     

背景介紹：The protein encoded by this gene is a cytoplasmic membrane-associated protein that indirectly coordinates endocytic membrane traffic with the actin assembly machinery. In addition, the encoded protein may regulate the formation of clathrin-coated vesicles and could be involved in synaptic vesicle recycling. This protein has been shown to interact with dynamin, CDC42, SNAP23, SNAP25, SPIN90, EPS15, EPN1, EPN2, and STN2. Multiple transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been characterized so far. 

產(chǎn)品規(guī)格：50ul；100ul；200ul；1ml，*

抗體來源 Rabbit or Mouse

克隆類型 Polyclonal or Monoclonal

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性    狀 Lyophilized or Liquid
濃    度 1mg/ml

Anti-Intersectin 1抗體；交叉蛋白1抗體產(chǎn)品信息：

SABC法

- 脫蠟、水化

- PBS洗2次各5分鐘

- 用蒸餾水或PBS配制新鮮的3% H2O2 ，室溫封閉5-10分鐘，用蒸餾水洗3次

- 抗原修復(fù)

- PBS洗5分鐘

- 滴加正常山羊血清封閉液，室溫20分鐘，甩去多余液體

- 滴加Ι抗50ul,室溫靜置1小時或4℃過夜或37℃1小時

- PBS洗3次各2分鐘

- 滴加*化Ⅱ抗，20℃-37℃ 20分鐘

- PBS洗3次各2分鐘

- 滴加試劑SABC 20℃-30℃ 20分鐘

- PBS洗4次各5分鐘

- DAB顯色：試劑盒或自配顯色劑顯色

- 脫水、透明、封片、鏡檢

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