草業(yè)科學(xué)-葉綠素?zé)晒獬上裣到y(tǒng)Weed-PAM

基于功能的IMAG-PAM，改進(jìn)升級(jí)后適用于野外雜草，草坪等光合作用研究

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主要功能

l 成像功能：對(duì)Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red等至少17種參數(shù)進(jìn)行成像分析。測(cè)定調(diào)節(jié)性能量耗散Y(NPQ)，反映植物光保護(hù)能力，測(cè)定非調(diào)節(jié)性能量耗散Y(NO)，反映植物光損傷程度。

l 程序測(cè)量功能：可程序測(cè)量熒光誘導(dǎo)曲線(xiàn)、快速光曲線(xiàn)和暗弛豫，也可手動(dòng)測(cè)量；在測(cè)量過(guò)程中能自動(dòng)分析所有熒光參數(shù)的變化趨勢(shì)

l AOI功能：可在測(cè)量前或測(cè)量后任意選擇感興趣的區(qū)域（AOI），程序?qū)⒆詣?dòng)對(duì)選擇的AOI的數(shù)據(jù)進(jìn)行變化趨勢(shì)分析，并在報(bào)告文件中顯示相關(guān)AOI的數(shù)據(jù)。所有報(bào)告文件中顯示的數(shù)據(jù)都可導(dǎo)出到EXCEL文件中。

l 成像異質(zhì)性分析功能：對(duì)任意參數(shù)任意時(shí)間的成像，可在圖像上任意選取兩點(diǎn)，軟件自動(dòng)對(duì)兩點(diǎn)間的數(shù)據(jù)進(jìn)行橫向異質(zhì)性分析，并可導(dǎo)出到EXCEL文件中。

l 成像數(shù)據(jù)范圍分析功能：對(duì)任意參數(shù)任意時(shí)間的成像，可分析任意兩個(gè)熒光數(shù)值之間有多少個(gè)像素點(diǎn)，多少面積（cm2）。

l 突變株篩選功能：可跟據(jù)成像結(jié)果快速篩選光合、產(chǎn)氫/油、抗逆（抗鹽、抗旱、抗病等）等突變株。

l 吸光系數(shù)測(cè)量功能：快速測(cè)量葉片的吸光系數(shù)。吸光系數(shù)測(cè)量光源： 16個(gè)紅光（650 nm）和16個(gè)近紅外（780 nm）LED，用于測(cè)量植物葉片或藻類(lèi)樣品PAR吸光系數(shù)。

成像參數(shù)

l Fo, Fm, F, Ft, Fm', Fv/Fm, Y(II), qL, qP, qN, NPQ, Y(NPQ), Y(NO), ETR, Abs, NIR和Red等

技術(shù)參數(shù)

Maxi探頭（藍(lán)光版）藍(lán)色LED，450 nm

Maxi探頭（紅光版）紅色LED，650 nm

Mini探頭（藍(lán)光版）藍(lán)色LED，460 nm

Mini探頭（紅光版）紅色LED，620 nm

應(yīng)用領(lǐng)域

l 光合作用研究：可以在相同的條件下同時(shí)對(duì)大量樣品進(jìn)行成像

l 植物病理學(xué)：病斑部位（包括肉眼不可見(jiàn)時(shí)）成像以及病斑擴(kuò)散的時(shí)空動(dòng)力學(xué)

l 植物脅迫生 理學(xué)：肉眼不可見(jiàn)脅迫損傷的早期檢測(cè)

l 遺傳育種：出苗后大規(guī)?？焖俸Y選高光合/抗旱/抗熱/抗凍/抗病等植株

l 突變株篩選：快速篩選模式植物的光合突變株、抗逆突變株、產(chǎn)氫微藻突變株等

l 分子生物學(xué)：宏觀水平上檢測(cè)樣品的綠色熒光蛋白（GFP）熒光

l 其它多種擴(kuò)展研究

參考文獻(xiàn)

數(shù)據(jù)來(lái)源：光合作用文獻(xiàn) Endnote 數(shù)據(jù)庫(kù)，更新至 2021 年 1 月，文獻(xiàn)數(shù)量超過(guò) 10000 篇

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