pco.flim相機(jī)系統(tǒng)是采用Two-Tap CMOS成像芯片的熒光壽命成像相機(jī)。相機(jī)的像素和激發(fā)光間的同步使100皮秒至100微秒間的熒光衰減時(shí)間的分析成為可能。在1008 x 1008像素分辨率上，pco.flim最多可以讀取45個(gè)雙幀/秒，且可支持5 kHz  -  40 MHz頻率范圍的調(diào)制。

其C-mount接口可以使改相機(jī)系統(tǒng)輕松地連接到任何顯微鏡或鏡頭。此外，其USB 3.0接口可讓您將相機(jī)連接到各種計(jì)算機(jī)。這會(huì)為您的實(shí)驗(yàn)和研究節(jié)省了大量的精力和成本。

pco.flim激光器是一種均勻照明的光源，設(shè)計(jì)用于pco.flim。它的數(shù)字調(diào)制頻率范圍為0  -  250 MHz。您也可以訂購pco.flim激光用于寬場(chǎng)熒光顯微鏡照明，或者用于全內(nèi)反射熒光（TIRF）顯微鏡，光片熒光顯微鏡（LSFM）和共焦旋轉(zhuǎn)盤顯微鏡。

您可在多種不同波長和輸出功率的激光。如果您樣本的發(fā)光壽命在幾納秒的范圍內(nèi)，則選擇激光作為光源使至關(guān)重要的。如果您使用的是尼康顯微鏡，我們的激光也提供相匹配的尼康雙安全快門選項(xiàng)。元奧儀器是德國PCO.AG公司在中國區(qū)的指定代理商，負(fù)責(zé)德國PCO.AG產(chǎn)品在中國市場(chǎng)的推廣、銷售、技術(shù)支持等工作，為客戶提供性價(jià)比高的產(chǎn)品。 歡迎您！

主要特點(diǎn)：

• 100 ps到100µs的壽命測(cè)量

• 調(diào)制頻率從5千赫茲到40兆赫

• 500千赫茲到40兆赫外部調(diào)制信號(hào)

• 正弦/矩形的調(diào)制信號(hào)波形

• 像素分辨率1008 x 1008

• 頻域FLIM

• USB 3.0接口

• 量子效率39%

• 動(dòng)態(tài)范圍1000:1

• 讀出噪音45 e- rms

• 90幀每秒 (2 tap讀出)

• 可選曝光時(shí)間為10ns到10秒

• 無振動(dòng)水冷

• 特殊的測(cè)量與分析軟件

pco.flim

luminescence lifetime imaging camera

The pco.flim is the first Frequency Domain FLIM camera using a two tap CMOS sensor. Synchronized modulation of pixels and stimulated light enables you the analysis of luminescence decay times in the range of 100 ps – 100 µs.

With its 1008 x 1008 pixels resolution the pco.flim reads out 45 double frames/s at a max. You can use it for a modulation frequency range of 5 kHz – 40 MHz.

Using C-mount as standard the system is easy to connect to any microscope or lens. Further, the USB 3.0 interface lets you connect the camera to all kinds of computers. This saves you significant efforts and costs for operation and research.

The pco.flim laser is a homogeneously illuminating light source designed for use with the pco.flim. It features a digital modulation frequency range of 0 – 250 MHz. You can either order the pco.flim laser for widefield epifluorescence microscope illumination, or for Total-Internal-Reflection-Fluorescence (TIRF) microscopy, light sheet fluorescence microscopy (LSFM) and confocal spinning disk microscopy.

Choose between a wide range of different wavelengths and light output powers. The laser is ideally suited if your relevant luminescence lifetimes are in the range of a few nanoseconds. In case you are using a Nikon microscope, the Nikon double-safety-shutter option is also available

• pco.flim laser as light source for epifluorescence measurements

• selctable laser wavelengths and light output powers

• 100 ps – 100 µs lifetimes measurable

• 5 kHz – 40 MHz modulation frequencies

• 500 kHz – 40 MHz external modulation signals

• modulation signal shape sinusoidal / rectangular

• 1008 x 1008 pixel resolution

• frequency domain FLIM

• USB 3.0 interface

• 39 % quantum efficiency

• 1000:1 dynamic range

• 45 double frames/s (2 tap readout)

• 1 ms to 2 s selctable exposure times

• vibration-free water cooling

• special software for measurement and analysis

注：產(chǎn)品信息若有變更恕不另行通知  / 德國PCO公司中國指定代理商---元奧儀器

技術(shù)參數(shù)：

芯片Qe圖：

pco.flim 熒光壽命成像系統(tǒng)裝配圖：

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應(yīng)用案例：

Figure 1: The left photo shows the fluorescence intensity of HEK-293 cells which expressed a CFP/DJ-1 protein as control of a FRET experiment. The middle image shows the phase angle derived distribution of fluorescence lifetimes in the range of 0 – 4 ns (imageJ LUT 16 colors, colorbar 0 – 4 ns) which has been masked by an intensity filter. The range of lifetimes around 2 ns was found in all of the 26 cells, which have been measured and showed about 10% FRET efficiency compared to the pure CFP expression. The right image shows the lifetime distribution image weighted by the fluorescence intensity image (the color images are false color coded using the same colorbar and LUT) without mask (courtesy of Prof. Dr. F.S. Wouters and Dr. G. Bunt, University Medicine G?ttingen).
Figure 5: A Thermofisher FluoCells® prepared slide, which contains a section of mouse kidney stained with a combination of fluorescent dyes. The visible marker is Alexa Fluor® 488 wheat germ agglutinin, a green-fluorescent lectin, which was used to label elements of the glomeruli and convoluted tubules, which were excited with 488 nm at a modulation frequency of 30 MHz. The left image shows the fluorescence intensity of the mouse kidney sample (20x air objective). The middle image shows the phase angle derived distribution of fluorescence lifetimes of the Alexa Fluor 488® in the range of 1.5 – 3.5 ns (NIS Elements, colorbar 1.5 – 3.5 ns). The right image shows the lifetime distribution image weighted by the fluorescence intensity image
Seemlesly integrated ino Nikon NIS Elements AR software to use the pco.flim camera for homodyne frequency domain fluorescence lifetime imaging with all referencing and phasor plot feedback.	Endogenous fluorescence of a Convallaria (lily of the valley) slice sample. The image shows the endogenous fluorescence lifetime distribution derived from the measured phase angle in false color coding and weighted by the fluorescence intensity. The displayed lifetimes range from 0.5 - 4 ns.	HEK-293 cells co-expressing a fusion protein with Cyan Fluorescent Protein (CFP) and with Yellow Fluorescent Protein (YFP). Dimerization of this protein is detected by FRET as judged by the reduction in CFP lifetime. The image shows the fluorescence lifetime distribution derived from the measured phase angle in false color coding and weighted by the fluorescence intensity. The displayed range is from 0 – 4 ns (see color bar, courtesy of Prof. Dr. F.S. Wouters and Dr. G. Bunt, University Medicine G?ttingen).

注：產(chǎn)品信息若有變更恕不另行通知  / 德國PCO公司中國指定代理商---元奧儀器